T1200

Anti-Tal (FQ-17) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-Tsg101-associated ligase
• MDL-Nummer: MFCD06798551
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 84 kDa
• Speziesreaktivität: human, mouse, rat
• Methode(n): immunoprecipitation (IP): 20-40 μg using mouse brain S1 cytosolic fraction; indirect immunofluorescence: 10-20 μg/mL using mouse fibroblast NIH3T3 cell line; microarray: suitable; western blot: 2-4 μg/mL using whole extract of human kidney 293 cells expressing human Tal
• UniProt-Hinterlegungsnummer: Q6UWE0
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... LRSAM1(90678) ; mouse ... Lrsam1(227738)

Beschreibung

Allgemeine Beschreibung

The gene LRSAM1 (leucine-rich repeat and sterile a motif-containing protein 1) is mapped to human chromosome 9q33.3. The protein is predicted to contain a leucine-rich repeat (LRR), an ezrin-radixin-moezin (ERM) domain, a coiled-coil (CC) region, a SAM (sterile α motif) domain and a carboxyl-terminal C3HC4-type RING (really interesting new gene) finger domain.

Immunogen

synthetic peptide encoding amino acids 5-21 located near the N-terminus of human Tal, conjugated to KLH. This sequence is identical in mouse and rat Tal.

Biochem./physiol. Wirkung

LRSAM1 (leucine-rich repeat and sterile a motif-containing protein 1) is an E3 ligase. It binds and ubiquitinates Tsg101 (tumor susceptibility gene 101), inactivating Tsg101-mediated sorting of epidermal growth factor receptors and viral proteins. It also protects cytoplasm from invasive pathogens by participating in ubiquitination associated with intracellular bacteria. Mutations in LRSAM1 are linked with Charcot-Marie-Tooth disease.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.