SRP5340

BCL2, GST tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(e): Bcl-2
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥70% (SDS-PAGE)
• Form: buffered aqueous glycerol solution
• Mol-Gew.: ~51 kDa
• NCBI-Hinterlegungsnummer: NM_000633
• Anwendung(en): cell analysis
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... BCL2(596)

Beschreibung

Allgemeine Beschreibung

BCL2 gene encodes an integral inner mitochondrial membrane protein that acts as an antiapoptotic protein. The protein BAD can antagonize both the cell cycle and antiapoptotic functions of BCL2 through binding to the BH3 domain.[1] Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. BCL2 is phosphorylated on specific serine/threonine residues within the unstructured loop in response to diverse stimuli and such phosphorylation has been associated with the loss of the biological function of BCL2.[2]

It is a target gene of nuclear factor κB (NF-κB). The gene encoding this protein is localized on human chromosome 18q21. B-cell lymphoma 2 (BCL-2) inhibits apoptosis in cells and has been shown to be upregulated in adenomas.[3][4][5]

Physikalische Form

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Angaben zur Herstellung

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable