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SRP5047

Sigma-Aldrich

MEKK1 (664-1512), active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(e):

MAP3K1, MAPKKK1, MEKK

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.32

Rekombinant

expressed in baculovirus infected Sf9 cells

Produktlinie

PRECISIO® Kinase

Assay

≥70% (SDS-PAGE)

Form

buffered aqueous glycerol solution

Spezifische Aktivität

697-943 nmol/min·mg

Mol-Gew.

~155 kDa

NCBI-Hinterlegungsnummer

NM_005921

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

human ... Map3K1(4214)

Allgemeine Beschreibung

MEKK1 or MEK kinase, is a serine/threonine kinase that is downstream of mitogenic and metabolic stimuli, including insulin and many growth factors. MEKK1 functions not only as an upstream activator of ERK and JNK through its kinase domain, but also as an E3 ligase through its PHD domain, providing a negative regulatory mechanism for decreasing ERK1/ERK2 activity by ubiquitination and degradation. MEKK1 -/- embryonic stem cells from mice show loss or altered responses of JNK to microtubule disruption and cold stress. Furthermore, activation of JNK is lost and that of ERK is diminished in response to hyperosmolarity and serum factors in MEKK1 -/- cells.

Physikalische Form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Angaben zur Herstellung

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Rechtliche Hinweise

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

T Yujiri et al.
Science (New York, N.Y.), 282(5395), 1911-1914 (1998-12-04)
Targeted disruption of the gene encoding MEK kinase 1 (MEKK1), a mitogen-activated protein kinase (MAPK) kinase kinase, defined its function in the regulation of MAPK pathways and cell survival. MEKK1(-/-) embryonic stem cells from mice had lost or altered responses
Zhimin Lu et al.
Molecular cell, 9(5), 945-956 (2002-06-07)
ERK1/2 MAP kinases are important regulators in cellular signaling, whose activity is normally reversibly regulated by threonine-tyrosine phosphorylation. In contrast, we have found that stress-induced ERK1/2 activity is downregulated by ubiquitin/proteasome-mediated degradation of ERK1/2. The PHD domain of MEKK1, a

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