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SRP5016

Sigma-Aldrich

CK1 γ 2, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(e):

CK1G2, CSNK1G2

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.32

Rekombinant

expressed in baculovirus infected Sf9 cells

Produktlinie

PRECISIO® Kinase

Assay

≥70% (SDS-PAGE)

Form

buffered aqueous glycerol solution

Spezifische Aktivität

25-35 nmol/min·mg

Mol-Gew.

~73 kDa

NCBI-Hinterlegungsnummer

NM_001319

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

human ... CSNK1G2(1455)

Allgemeine Beschreibung

Casein kinase 1 γ 2 (CK1γ2) is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. It is a ubiquitously expressed cytoplasmic kinase. The gene encoding this protein is localized on human chromosome 19p13.3.

Biochem./physiol. Wirkung

Casein kinase 1 γ 2 (CK1γ2) can interact and phosphorylate the metastatic tumor antigen 1 short form (MTA1s) co-localizing it in the cytoplasm. Phosphorylated MTA1s then sequesters the estrogen receptor-α in the cytoplasm of breast cancer cells. CK1γ2 hyperphosphorylates the ceramide transfer protein CERT leading to a decrease in de novo sphingomyelin synthesis. The reduction in synthesis of sphingomyelin is reversed by the expression of CERT mutants that are not hyperphosphorylated.

Physikalische Form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Angaben zur Herstellung

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Rechtliche Hinweise

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Role for casein kinase 1 in the phosphorylation of Claspin on critical residues necessary for the activation of Chk1
Zheng Meng
Molecular Biology of the Cell (2011)
Cloning and Chromosomal Mapping of Human Casein Kinase I ?2 (CSNK1G2)
Aya N
Genomics Data (1997)
Sphingolipid Metabolism and Interorganellar Transport: Localization of Sphingolipid Enzymes and Lipid Transfer Proteins
Toshiyuki Yamaji and Kentaro Hanada
Traffic (2014)
MTA family of coregulators in nuclear receptor biology and pathology
Bramanandam M
Nuclear Receptor Signaling (2007)
Sandip K Mishra et al.
Oncogene, 23(25), 4422-4429 (2004-04-13)
Recent studies have shown that metastasis-associated protein-1 short form (MTA1s) - metastatic tumor antigen 1 short form sequesters estrogen receptor-alpha (ER-alpha) in the cytoplasm of breast cancer cells. Using a yeast two-hybrid screening to clone MTA1s-interacting proteins, we identified casein
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