SRP3216

KC (CXCL1) from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(e): CXCL1, GRO1, GROa, NAP-3
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: mouse
• Rekombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Form: lyophilized
• Mol-Gew.: 7.8 kDa
• Verpackung: pkg of 20 μg
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: <0.1 EU/μg endotoxin, tested
• Farbe: off-white to yellow
• UniProt-Hinterlegungsnummer: P12850
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... CXCL1(14825)

Beschreibung

Allgemeine Beschreibung

C-X-C motif chemokine receptor 1 (CXCL1) is also known as keratinocyte-derived chemokine (KC). It is part of the glutamate-leucine-arginine (ELR)-containing CXC chemokine family. Recombinant Murine KC (CXCL1) is a 7.8kDa protein consisting of 72 amino acids including the ‘ELR′ motif common to the CXC chemokine family that bind to CXCR1 or CXCR2.

Anwendung

C-X-C motif chemokine receptor 1 (CXCL1) from mouse has been used in transwell assay for chemotaxis.

Biochem./physiol. Wirkung

All three isoforms of growth-related protein (GRO) are CXC chemokines that can signal through C-X-C motif chemokine receptors 1 or 2 (CXCR1 or 2). The GRO proteins chemoattract and activate neutrophils and basophils. It has a role in host defense against polymicrobial sepsis, upregulation of intercellular adhesion molecule 1 (ICAM-1) and migration of neutrophils. The protein also activates mitogen-activated protein kinases (MAPKs), pro-inflammatory proteins and nuclear factor-κB (NF-κB).

Sequenz

APIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK

Physikalische Form

Lyophilized with no additives.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable