SRP0209

PRL-2 (2-167) Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonym(e): OV-1, PRL-2, PTP4A2, Protein tyrosine phosphatase type IVA, member 2, Protein-tyrosine phosphatase of regenerating liver 2
• UNSPSC-Code: 51111800
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥90% (SDS-PAGE)
• Form: aqueous solution
• Mol-Gew.: 19.1 kDa
• Verpackung: pkg of 50 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Konzentration: >0.02 mg/mL
• NCBI-Hinterlegungsnummer: NM_080391
• UniProt-Hinterlegungsnummer: Q12974
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... PTP4A2(8073)

Beschreibung

Allgemeine Beschreibung

Human PRL 2 (GenBank Accession No. NM_080391), (amino acids 2-167) with N-terminal HIS tag, MW = 19.1 kDa, expressed in an E. coli expression system.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Einheitendefinition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.