SAB4200720

Anti-β-Catenin antibody, Mouse monoclonal

clone 15B8, purified from hybridoma cell culture

• Synonym(e): Anti-CTNNB1, Anti-armadillo
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Antikörperform: purified from hybridoma cell culture
• Antikörper-Produkttyp: primary antibodies
• Klon: 15B8, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~94 kDa
• Speziesreaktivität: chicken, mouse, human, rat, monkey, hamster, bovine, canine
• Konzentration: ~1.0 mg/mL
• Methode(n): immunoblotting: 0.25-0.5 μg/mL using Madin-Darby bovine kidney (MDBK) cell line extract; immunofluorescence: 5-10 μg/mL using human breast adenocarcinoma MCF7 cells; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: P35222
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... CTNNB1(1499) ; mouse ... Ctnnb1(12387) ; rat ... Ctnnb1(84353)

Beschreibung

Allgemeine Beschreibung

Anti-β-Catenin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 15B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant chicken β-Catenin. β-Catenin, also known as Catenin βfamily.

Immunogen

recombinant chicken ß-Catenin

Anwendung

Anti-β-Catenin antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry
• immunoprecipitation

Biochem./physiol. Wirkung

β-Catenin plays a dual function in the cell including cell-cell adhesion and as a component of the Wnt (wingless) signaling pathway. In adherens junctions, β-Catenin associates with the cytoplasmic domain of the cadherin family adhesion receptors, anchoring them to the actin cytoskeleton and thus acting as structural protein. β-Catenin interacts with T cell factor (TCF)- family transcription factors to activate target genes. Wnt/β-catenin signaling interacts with several cellular pathways in context-dependent ways, including JakSTAT (Janus kinase−Signal transducer and activator of transcription) cytokine, TGFβ (transforming growth factor−β), DNA damage and the Hippo signaling pathways. Furthermore, β-Catenin is involved in embryonic morphogenesis and tumorigenesis and is tightly regulated by phosphorylation and further degradation by the ubiquitin-proteasome system.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable