SAB4200413

Monoclonal Anti-LIN28 antibody produced in mouse

clone L28.5, tissue culture supernatant

• Synonym(e): Monoclonal Anti-CSDD1, Monoclonal Anti-LIN28A, Monoclonal Anti-ZCCHC1
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Biologische Quelle: mouse
• Konjugat: unconjugated
• Antikörperform: tissue culture supernatant
• Antikörper-Produkttyp: primary antibodies
• Klon: L28.5, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~28 kDa
• Speziesreaktivität: mouse, human
• Methode(n): western blot: 1:8,000-1:16,000 using extracts of Ntera-2 cells.
• Isotyp: IgM
• UniProt-Hinterlegungsnummer: Q9H9Z2
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... LIN28A(79727) ; mouse ... Lin28a(83557)

Beschreibung

Allgemeine Beschreibung

LIN28 belongs to a family of RNA-binding proteins that are mainly expressed in embryonic stem cells.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminus of human LIN28A. The isotype is determined using a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Anwendung

Monoclonal Anti-LIN28 antibody produced in mouse is suitable for immunoblotting at a working dilution of 1:8000 to 1:16000. For immunofluorescence, a dilution of 1:1500 to 1:3000 is recommended.

Monoclonal Anti-LIN28 antibody produced in mouse specifically recognizes C-terminus of human LIN28A.

Biochem./physiol. Wirkung

The structure of LIN28 includes RNA-binding domains, an N-terminal cold-shock domain and a C-terminal Zn-knuckle domain. It inhibits the biogenesis of let-7 miRNA and modulates the translation of target mRNA. The expression of LIN28 is linked to the cellular processes of development, differentiation, oncogenesis and glucose metabolism. It is also linked to the resistance of breast cancer cells to radiation effects.

Physikalische Form

Solution containing 15 mM sodium azide.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable