SAB4200340

Anti-Golph3 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(e): Anti-GOPP1, Anti-GPP34, Anti-Golgi peripheral membrane protein 1, 34 kDa, Anti-Golgi phosphoprotein 3 (coat-protein), Anti-Golgi-associated protein, Anti-MIDAS, Anti-Mitochondrial DNA absence factor
• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~34 kDa
• Speziesreaktivität: human
• Konzentration: ~1.0 mg/mL
• Methode(n): immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells.; western blot: 0.1-0.2 μg/mL using whole extracts of HEK-293 cells over-expressing human Golph3.
• UniProt-Hinterlegungsnummer: Q9H4A6
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... GOLPH3(64083) ; mouse ... Golph3(66629)

Beschreibung

Allgemeine Beschreibung

Golgi phosphoprotein 3 (Golph3) is a peripheral membrane protein of the Golgi stack that localizes to the trans-Golgi network. It is conserved from yeast to human. The GOLPH3 gene is mapped to the human chromosome 5p13.3 location.

Spezifität

Anti-Golph3 (C-terminal) recognizes human Golph3.

Immunogen

peptide corresponding to the C-terminal region of human Golph3, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey and canine.

Anwendung

Anti-Golph3 (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem./physiol. Wirkung

Golgi phosphoprotein 3 (Golph3) is a phosphatidylinositol-4-phosphate (PtdIns(4)P) binding protein required for Golgi function. It plays a vital role in Golgi trafficking and morphology by interacting with the unconventional myosin 18A (MYO18A) linking Golgi membranes to the F-actin cytoskeleton. GOLPH3 is an oncogene that is commonly targeted for amplification in human cancer and cancer cell lines. Furthermore, Golph3 overexpression is correlated with the hyperactivation of the mammalian target of rapamycin (mTOR) signaling in human cells.

Physikalische Form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable