R3654

Anti-RbAp48 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-Retinoblastoma-binding protein
• MDL-Nummer: MFCD02686588
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 48 kDa
• Speziesreaktivität: human
• Methode(n): indirect immunofluorescence: 1-2 μg/mL using HeLa cells fixed with paraformaldehyde-Triton; western blot: 0.5-1 μg/mL using MCF7 nuclear extracts
• UniProt-Hinterlegungsnummer: Q09028
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... RBBP4(5928) ; mouse ... Rbbp4(19646) ; rat ... Rbbp4(313048)

Beschreibung

Allgemeine Beschreibung

Histone binding protein (RbAp48, also known as also known as RBBP4 or NURF55) contains 425 amino acids, tryptophan-aspartic acid (WD)-domain containing protein isolated as a retinoblastoma binding protein (RB1).

Immunogen

synthetic peptide corresponding to amino acids 1-14 of human RbAp48, conjugated to KLH via a C-terminal added cysteine residue. The immunizing peptide is conserved in human, mouse, and rat.

Anwendung

Anti-RbAp48 antibody produced in rabbit has been used in immunoprecipitation and western blot analysis. It may also be used in immunofluorescence.

Biochem./physiol. Wirkung

RbAp48 is found in association with chromatin remodeling complexes Sin3A/histone deacetylase (HDAC) and NURD (nucleosomal remodeling and deacetylation complex).

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)