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PIC0004

Sigma-Aldrich

Protease Inhibitor Cocktail

ReadyShield® ready-to-use solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, for use in purification of Histidine-tagged proteins, Non-freezing solution

Synonym(e):

Protease inhibitor cocktail

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.28

product name

ReadyShield® Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, Non-freezing solution

Beschreibung

Purification of Histidine-Tagged Proteins

Form

ready-to-use solution

Qualität

200

Verpackung

pkg of 1 mL (in amber polypropylene vial)
pkg of 5 mL (in amber polypropylene vial)

Lagertemp.

−20°C

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Allgemeine Beschreibung

This particular protease inhibitor cocktail omits chelators (e.g., EDTA), to optimize its use for purification of histidine-tagged proteins.1 It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. The cocktail has been tested on extracts from E. coli and insect cells This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0004 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P8849.

Leistungsmerkmale und Vorteile

  • Does not freeze in storage for quick, convenient use.
  • Tailored for use with Histidine fusion tagged proteins.
  • Suggested as 100 X solution.
  • Reclosable screw-top vials.
  • Can be stored in cooler for approx. 1 month or room temperature for a week.

Angaben zur Herstellung

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 100 ml of lysate from 20 g (wet weight) of E. coli cells or 10 g (wet weight) of baculovirus-infected insect (Spodoptera frugiperda) cells.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Rechtliche Hinweise

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

H-Sätze

H315,H319

Gefahreneinstufungen

Eye Irrit. 2 - Skin Irrit. 2

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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