P0083

PRMT1 from rat

recombinant, expressed in E. coli, ≥90% (SDS-PAGE), buffered aqueous solution

• Synonym(e): HMT1-like 2, HRMT1L2, Heterogenious nuclear ribonucleoprotein methyltransferase 1-like 2, IR1B4, Interferon receptor 1-bound protein 4, Protein arginine N-methyl transferase 1
• UNSPSC-Code: 51111800
• NACRES: NA.32

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 200
• Assay: ≥90% (SDS-PAGE)
• Form: buffered aqueous solution
• UniProt-Hinterlegungsnummer: Q63009
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Angaben zum Gen: rat ... Prmt1(60421)

Beschreibung

Biochem./physiol. Wirkung

Methyl transferases catalyze the addition of methyl groups to nitrogen, carbon, sulfur, and oxygen atoms of small molecules, lipids, proteins, and nucleic acids. Eight mammalian protein arginine methyltransferases (PRMT) have been identified. PRMT1 is the predominant member of the methyl transferases, which catalyzes the protein arginine N-methylation reactions. PRMT1 is implicated in various cellular processes including: transcription, RNA processing, and signal transduction.

Einheitendefinition

The specific activity is ≥ 0.1 nmol/mgP/min. measured by ³H-AdoMet incorporation into histone for 30 minues at 30 °C.

Physikalische Form

Solution of 50 mM Tris, pH 7.6, 5 mM DTT, 0.2% IGEPAL^® CA-630, 150 mM NaCl, and 30% glycerol (w/v).

Hinweis zur Analyse

The N-methyltransferase activity is determined by detecting the level of radiolabel transfer from ³H-AdoMet (Methyl donor) to histone (Cat. No. H4380), which is arginine rich (methyl acceptor).

Rechtliche Hinweise

IGEPAL is a registered trademark of Solvay

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)