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OGS3162

PSF-OXB20-NH2-PHOA - PHOA SECRETION PLASMID

Name: PSF-OXB20-NH2-PHOA - PHOA SECRETION PLASMID
Brand: SIGMA

plasmid vector for molecular cloning

Synonym(e):

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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Alle Fotos(2)

About This Item

UNSPSC-Code:

12352200

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pPolh-MAT-Tag^®-1 Transfer Vector

Rekombinant

expressed in E. coli

Form

buffered aqueous solution

Mol-Gew.

size 3911 bp

Bakterienauswahl

kanamycin

Replikationsursprung

pUC (500 copies)

Peptidspaltung

no cleavage

Lage der Peptid-Tags

N-terminal

Promoter

Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial

Reportergen

none

Sekretionssignal

PhoA

Versandbedingung

ambient

Lagertemp.

−20°C

Allgemeine Beschreibung

This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.

About the Peptide Tag:This plasmid contains an PhoA secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases. .

Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.

Sequenz

To view sequence information for this product, please visit the product page

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Lagerklassenschlüssel

12 - Non Combustible Liquids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Dokumente

PSF-OXB20-NH2-PHOA - PHOA SECRETION PLASMID

plasmid vector for molecular cloning

About This Item

Empfohlene Produkte

Eigenschaften

Rekombinant

Form

Mol-Gew.

Bakterienauswahl

Replikationsursprung

Peptidspaltung

Lage der Peptid-Tags

Promoter

Reportergen

Sekretionssignal

Versandbedingung

Lagertemp.

Beschreibung

Allgemeine Beschreibung

Sequenz

Hinweis zur Analyse

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Sicherheitshinweise

Lagerklassenschlüssel

Flammpunkt (°F)

Flammpunkt (°C)

Dokumentation

Analysenzertifikate (COA)

Besitzen Sie dieses Produkt bereits?

Peer-Reviewed-Artikel

Technischer Dienst