OGS1634
PSF-TEF1-NH2-HA-TEV - N-TERMINAL HA TAG YEAST PLASMID
plasmid vector for molecular cloning
Synonym(e):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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About This Item
UNSPSC-Code:
12352200
NACRES:
NA.85
Markierung
Hemagglutinin A (HA) tagged
Form
buffered aqueous solution
Mol-Gew.
size 6763 bp
Bakterienauswahl
kanamycin
Replikationsursprung
2Micron
pUC (500 copies)
Peptidspaltung
TEV
Lage der Peptid-Tags
N-terminal
Promotor
Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast
Reportergen
none
Versandbedingung
ambient
Lagertemp.
−20°C
Hefeauswahl
uracil
Allgemeine Beschreibung
This plasmid is designed to express tagged proteins in yeast cells (Saccharomyces cerevisiae). The plasmid contains an auxotrophic Uracil selection expression cassette (URA3) that allows for the positive selection of yeast that are deficient in the URA3 gene (YEL021W). This is typically achieved by growing the yeast in minimal media that is reconstituted with the essential amino acids and nucleotides but excluding Uracil.
About the Peptide Tag:This plasmid contains an n-terminal Influenza Hemagglutinin (HA) epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: YPYDVPDYA.
About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a TEV cleavage tag. The protein sequence of the cleavage tag is: ENLYFQG. Cleavage occurs between the Glu and Gly residues. TEV is often reported to have better specificity for its recognition site compared to EKT Thrombin or Faxtor Xa.
Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.
About the Peptide Tag:This plasmid contains an n-terminal Influenza Hemagglutinin (HA) epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: YPYDVPDYA.
About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a TEV cleavage tag. The protein sequence of the cleavage tag is: ENLYFQG. Cleavage occurs between the Glu and Gly residues. TEV is often reported to have better specificity for its recognition site compared to EKT Thrombin or Faxtor Xa.
Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.
Sequenz
To view sequence information for this product, please visit the product page
Hinweis zur Analyse
To view the Certificate of Analysis for this product, please visit www.oxgene.com
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Lagerklassenschlüssel
12 - Non Combustible Liquids
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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