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Merck

MBD0010

Sigma-Aldrich

Inactivated Salmonella enterica

Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml

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1 ML
CHF 279.00

CHF 279.00


Voraussichtliches Versanddatum16. April 2025



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1 ML
CHF 279.00

About This Item

UNSPSC-Code:
41105500
NACRES:
NA.51

CHF 279.00


Voraussichtliches Versanddatum16. April 2025


Qualitätsniveau

Form

liquid

Konzentration

>10^8 bacteria/ml

Methode(n)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

Versandbedingung

dry ice

Lagertemp.

−20°C

Allgemeine Beschreibung

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses.1 Inactivated Salmonella enterica can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.Salmonella enterica serotype Typhimurium (also S. Typhimurium) is a rod-shaped gram-negative bacterium of the family Enterobacteriaceae. It is an important food-borne pathogen that in humans causes intestinal inflammation, diarrhea, and the presence of neutrophils in stool samples.2 Growth of S. enterica in the gut lumen causes depletion of the healthy microbial community.3 Infection with Salmonella can induce inflammation and increase the risk for inflammatory bowel disease (IBD)4

Read here how to use our standards to ensure data integrity for your microbiome research.

Anwendung

Inactivated bacteria are provided at >10^8 bacteria/ml concentration in TE buffer pH 8.0.It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS

Leistungsmerkmale und Vorteile

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Physikalische Form

Liquid -The inactivated bacteria is provided at >10^8 bacteria/ml in TE buffer pH 8.0

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Kim O Gradel et al.
Gastroenterology, 137(2), 495-501 (2009-04-14)
Various commensal enteric and potentially pathogenic bacteria may be involved in the pathogenesis of inflammatory bowel diseases (IBD). We compared the risk of IBD between a cohort of patients with documented Salmonella or Campylobacter gastroenteritis and an age- and gender-matched
Inna Sekirov et al.
Gut microbes, 1(1), 30-41 (2011-02-18)
Gastrointestinal infections involve an interactive tripartite relationship between the invading pathogen, the host, and the host's resident intestinal microbiota. To characterize the host inflammatory response and microbiota alterations during enteric salmonellosis, C57BL/6 mice were pre-treated with a low dose of
Fecal leukocytes in diarrheal illness.
J C Harris et al.
Annals of internal medicine, 76(5), 697-703 (1972-05-01)

Artikel

Turn to our Next-Gen Sequencing Oligos (NGSO) as a potential solution if you are experiencing high levels of adapter dimer formation with custom adapters sourced from other vendors.

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

Questions

  1. active salmonella와 비교하였을 때, inactivation 된 현 제품의 구조와 사이즈 변화가 있나요? (샘플 내 균 분리에 이용할 예정 입니다.)

    1 answer
    1. Salmonella is a rod-shaped bacteria generally 2–5 microns long by 0.5–1.5 microns wide. The inactivated form has not been modified and will fall into the published size range.

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