Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Wichtige Dokumente
MAK117
Glycerin-Assay-Kit
sufficient for 200 colorimetric or fluorometric tests
Synonym(e):
Glycerol-Assay-Kit, Glyzerin-Assay-Kit
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About This Item
Empfohlene Produkte
Verwendung
sufficient for 200 colorimetric or fluorometric tests
Anwendung(en)
cosmetics
environmental
food and beverages
Nachweisverfahren
colorimetric
fluorometric
Relevante Krankheit(en)
cancer
Versandbedingung
dry ice
Lagertemp.
−20°C
Allgemeine Beschreibung
Anwendung
Leistungsmerkmale und Vorteile
Eignung
Prinzip
Ähnliches Produkt
Lagerklassenschlüssel
10 - Combustible liquids
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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Is there any information available on how to prepare tissue for assay, considering that technical bulletin MAK117 does not include any recommendations on this matter?
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The general recommendation for tissue sample preparation is as follows:
1. Begin with 20-100 mg tissue and add 200-1000 uL ice-cold PBS.
2. Lysis can be achieved by homogenization (10-20 passes in a Dounce homogenizer on ice) or by sonication (preferably performed in an ice-water bath). The degree of tissue lysis can be checked under a microscope.
3. Centrifuge the homogenate at 14,000 g for 10 min.
4. Transfer the clear supernatant into a clean tube and use it for the assay.Helpful?
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Can serum glycerol content be analyzed or just plasma?
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Yes, this product can determine glycerol content in serum samples.
Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/260/409/mak117bul.pdfHelpful?
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How long are reagents stable at 2-8 C after initial thaw?
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The Assay Buffer, MAK117A, may be stored at 2–8 Celsius. For all other components, it is recommended to aliquot and freeze at - 20 deg C. Avoid repleated freeze/thaw cycles.
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