L9639

Ricin B chain from Ricinus communis (castor bean)

buffered aqueous solution

• CAS-Nummer: 96638-29-8
• MDL-Nummer: MFCD00166673
• UNSPSC-Code: 12352200

Eigenschaften

• Form: buffered aqueous solution
• Lagertemp.: 2-8°C

Beschreibung

Biochem./physiol. Wirkung

Ricin B chain has an affinity for galactose and saccharides containing nonreducing terminal β-galactosyl units. This affinity allows the B chain to bind to cell surface glycoconjugates. In the intact ricin toxin, the surface binding by the B chain allows the A chain to enter into the cell. The saccharide binding capability of ricin B chain is independent of the presence of the A chain.

Sonstige Hinweise

Ricinus communis toxin (also named RCA₆₀, RCA_II, Ricin D, or RCL III) is a highly toxic protein, mol. wt. ~60 kDa, composed of two chains, A and B, connected by a single disulfide bridge.

Qualität

Electrophoretically pure (SDS-PAGE)

Physikalische Form

Solution in 10 mM phosphate, pH 6.5, containing 0.15 M NaCl, 10 mM galactose, 0.5 mM dithioerythritol and 0.02% sodium azide

Angaben zur Herstellung

Highly purified by affinity chromatography

Hinweis zur Analyse

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg protein per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 °C.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable