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G5894

Sigma-Aldrich

Glutaminase aus E. coli

Grade VII, lyophilized powder, 500-1,500 units/mg protein

Synonym(e):

L-Glutamine amidohydrolase

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About This Item

CAS-Nummer:
9001-47-2
EC-Nummer:
3.5.1.2 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00131193
UNSPSC-Code:
12352204
NACRES:
NA.54

Typ

Grade VII

Qualitätsniveau

200

Form

lyophilized powder

Spezifische Aktivität

500-1,500 units/mg protein

Zusammensetzung

Protein, ~10% Lowry

Lagertemp.

−20°C

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Biochem./physiol. Wirkung

Glutaminase catalyzes the conversion of glutamine to glutamate.

Verlinkung

Chromatographically purified from Grade V

Einheitendefinition

One unit will deaminate 1.0 μmole of L-glutamine per min at pH 4.9 at 37 °C.

Physikalische Form

Lyophilized powder containing stabilizer and potassium succinate

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

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Felix List et al.
Chemistry & biology, 19(12), 1589-1599 (2012-12-25)
Nitrogen is incorporated into various metabolites by multifunctional glutamine amidotransferases via reactive ammonia generated by glutaminase hydrolysis of glutamine. Although this process is generally tightly regulated by subsequent synthase activity, little is known about how the glutaminase is inhibited in
Catherine Qiurong Pan et al.
FEBS letters, 586(17), 2674-2691 (2012-06-20)
The BNIP-2 and Cdc42GAP Homology (BCH) domains constitute a new and expanding family of highly conserved scaffold protein domains that regulate Rho, Ras and MAPK signaling, leading to cell growth, apoptosis, morphogenesis, migration and differentiation. Such versatility is achieved via
A Pieter J van den Heuvel et al.
Cancer biology & therapy, 13(12), 1185-1194 (2012-08-16)
One of the hallmarks of cancer is metabolic deregulation. Many tumors display increased glucose uptake and breakdown through the process of aerobic glycolysis, also known as the Warburg effect. Less studied in cancer development and progression is the importance of
Sonya B Dumanis et al.
Journal of neurochemistry, 124(1), 4-14 (2012-08-07)
Apolipoprotein E (APOE) genotype affects outcomes of Alzheimer's disease and other conditions of brain damage. Using APOE knock-in mice, we have previously shown that APOE-ε4 Targeted Replacement (TR) mice have fewer dendritic spines and reduced branching in cortical neurons. As
Natalie E Simpson et al.
Epigenetics, 7(12), 1413-1420 (2012-11-03)
The interplay of metabolism and epigenetic regulatory mechanisms has become a focal point for a better understanding of cancer development and progression. In this study, we have acquired data supporting previous observations that demonstrate glutamine metabolism affects histone modifications in
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