AFRC IAH-CC62

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Mouse x Mouse Hybridoma
• Verpackung: tube of 5 μg 99011334-DNA-5UG; pkg of vial of cells 99011334-1VL
• ImmunoDon: BALB/c mouse spleen
• Myelom: NS-1
• Reaktivität: Anti-CD26 (formerly WC10), bovine
• Methode(n): cell culture | mammalian: suitable
• Isotyp: IgG2b
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse x Mouse hybridoma

Beschreibung der Zelllinie

The hybridoma IAH-CC62 secretes antibodies reactive with bovine CD26 - this was formerly known as WC10. This non-lineage specific antigen is present on bovine leukocytes and has a MW of 115 kDa and 39kDa. Cells have been grown on mouse macrophage feeder cells using hypoxanthine and thymidine at concentractions appropriate for hybridomas. It is recommended to continue this until sufficient cells have been frozen.

Immunogen

Bovine thymocytes

Anwendung

Immunohistology, Flow cytometry, Immunoprecipation

Nährmedium

RPMI 1640 + 2 mM glutamine + HT supplement + 10% FBS

Subkultur-Routine

Maintain cultures between 3-9x 100,000 cells/ml; 5% CO₂; 37°C.

Sonstige Hinweise

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