LB205

98062329, human blood, Lymphoblastoid

• UNSPSC-Code: 41106514

Eigenschaften

• product name: LB205, 98062329
• Biologische Quelle: human blood
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Lymphoblastoid
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human lymphocytes, Xeroderma pigmentosum variant, EBV-transformed

Beschreibung der Zelllinie

Derived from mononuclear cells from the peripheral blood of a 62 year old male. The patient was diagnosed with Xeroderma pigmentosum variant, illustrating that the disease is heterogeneous. T cells from the original sample have been used for UV irradiation studies and hprt mutant frequency. The cell line has been established by transformation with EBV. Fibroblasts from the same individual are designated XP11 BR.

Anwendung

DNA repair deficiency studies, hprt mutation analysis

Nährmedium

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Subkultur-Routine

Maintain cultures between 3-9 x 100,000 cells/ml dilute every 1-2 days to 3 x 100,000 cells/ml 5%

Sonstige Hinweise

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