AFRC 56D2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Horse x Mouse/Horse Hybridoma
• Verpackung: tube of 5 μg 94120826-DNA-5UG; pkg of vial of cells 94120826-1VL
• ImmunoDon: Horse peripheral lymph node
• Myelom: AFRC 17D1
• Reaktivität: Anti-Haemagglutinin, influenza
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Horse peripheral lymph node x AFRC 17D1

Beschreibung der Zelllinie

The heterohybridoma AFRC 56D2 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 56D2 horse cells from the peripheral lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).

Immunogen

Influenza A equine 2 virus

Anwendung

Haemagglutinin detection

The isotype of the monoclonal antibody produced by this cell line is horse IgG.

Nährmedium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

Sonstige Hinweise

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