C2

94101906, rat liver, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: C2, 94101906
• Biologische Quelle: rat liver
• Wachstumsmodus: Adherent
• Karyotyp: Hyperdiploid, 52 Chromosomes
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat hepatoma [HGPRT-]

Beschreibung der Zelllinie

Full name Faof1C2 is a dedifferentiated variant of Fao. These cells continue to express tyrosine aminotransferase, but the liver-specific markers are mostly absent.

Anwendung

Gene expression analysis.

Nährmedium

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split semi-confluent cultures 1:50 i.e. seeding at 2x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 10x10,000,000,000 cells/10cm² dish.

Sonstige Hinweise

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