SK-N-AS

94092302, human nerve (bone marrow metastasis), Polygonal

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: SK-N-AS, 94092302
• Biologische Quelle: human nerve (bone marrow metastasis)
• Wachstumsmodus: Adherent
• Karyotyp: 46
• Morphologie: Polygonal
• Produkte: Not specified
• Rezeptoren: Vimentin
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): metastasis
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human neuroblastoma

Beschreibung der Zelllinie

Derived from a female patient with neuroblastoma from the metastasis of the bone marrow. Tumourigenic in nude mice.

Anwendung

Neuroblastoma differentiation studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 9
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 9.3
TPOX: 11,12
vWA: 16,17

Nährmedium

DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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