ATHOS

93122325, Porcine thyroid, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: ATHOS, 93122325
• Biologische Quelle: Porcine thyroid
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Thyroglobulin, plasminogen activator (u-PA)
• Rezeptoren: Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Porcine thyroid epithelial

Beschreibung der Zelllinie

The cell line was spontaneously derived from the porcine thyroid cell line PORTHOS (Sigma Catalogue number. 93122323) after withdrawal of thyroid stimulating hormone (TSH). The cells form follicle-like structures, have a high growth rate, a high glucose consumption and are TSH independent for growth. The expression of plasminogen activators has been reported and found to be higher in ATHOS cells compared to PORTHOS cells.

Anwendung

Mechanism of thyroid follicle reconstruction

Nährmedium

Click:RPMI (1:1) + 10μg/ml Insulin + 10nM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x100,000 cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO₂; 37°C.

Sonstige Hinweise

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