DK

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: dog kidney
• Verpackung: tube of 5 μg 93120836-DNA-5UG; pkg of vial of cells 93120836-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Canine kidney

Beschreibung der Zelllinie

Derived from the kidney cortex of a 6 week old Beagle of unknown sex. The cells support the growth of many viruses including influenza, parainfluenza-2, infectious bovine rhinotracheitis, canine hepatitis, adenovirus, vaccinia and VSV (Indiana strain).

Anwendung

DK cell line has been used to study the functional organization of the major late transcriptional unit of canine adenovirus type 2 (CAV-2).[1]

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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