BE10-Late

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: rat brain
• Verpackung: tube of 5 μg 93040120-DNA-5UG; pkg of vial of cells 93040120-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Plasminogen activator activity
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat, BDIX, foetal brain, malignant

Beschreibung der Zelllinie

Malignant cells produced on passaging BE10-Intermediate (Sigma Catalogue number. 93040119) beyond passage 40. The cells possess tissue plasminogen activator activity, are tumourigenic in BDIX rats and form colonies in agar.

Anwendung

Study of carcinogenesis, tumourigenicity studies

Nährmedium

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-2x100,000 cells/25cm² using 0.25% trypsin/EDTA; 10-15% CO₂; 37°C.

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.