PI.1 Ut

90111909, raccoon uterus, Fibroblast-like

• UNSPSC-Code: 41106514

Eigenschaften

• product name: PI.1 Ut, 90111909
• Biologische Quelle: raccoon uterus
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 38
• Morphologie: Fibroblast-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Racoon uterus

Beschreibung der Zelllinie

Derived from trypsinised uterine tissue of an adult female Racoon. Pl.1Ut was developed for studies of feline and canine viruses since the Racoon is one of the few animals which is susceptible to both.

Anwendung

Virus studies: feline and canine viruses

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm² by washing 3 times with 0.255 trypsin or trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan; maximum 90 passages expected. Growth rate is very slow.

Sonstige Hinweise

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