RR1022

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: rat unknown/unspecified
• Verpackung: tube of 5 μg 90102526-DNA-5UG; pkg of vial of cells 90102526-1VL
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 42
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat Amsterdam Schmidt-Ruppin sarcoma

Beschreibung der Zelllinie

Derived from a Rous sarcoma virus (Schmidt-Ruppin strain) induced tumour in an inbred Amsterdam rat. The cells are highly tumorigenic in new-born rats and also chickens. There is some evidence that the cell line is latently infected with the virus.

Anwendung

Tumourigenicity and virus studies: Rous sarcoma virus

Nährmedium

Medium 199 (HBSS) + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Sonstige Hinweise

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