NCTC 2071

90050803, mouse connective tissue, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: NCTC 2071, 90050803
• Biologische Quelle: mouse connective tissue
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 63
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse C3H/An connective tissue

Beschreibung der Zelllinie

Derivative of NCTC clone 929 and was established in chemically defined medium (NCTC 109). It was subsequently adapted to cysteine-free medium (NCTC 135). The cells have been used in nutritional, immunological and toxicological studies; nutritional, metabolic and enzyme studies; preservation and storage of viable tissue; fluid suspension culture methods and replicate culture techniques and quantitative cell culture methods.

Anwendung

Nutritional, immunological and toxicological studies

Nährmedium

NCTC 135 100% + 2mM Glutamine.

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 5x10,000 to 2x100,000 cells/cm² by scraping off the cells and resuspending into new flasks; 5% CO₂; 37°C. Freeze in 9% glycerol/91% FBS.

Sonstige Hinweise

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