153BR

90011808, human skin, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: 153BR, 90011808
• Biologische Quelle: human skin
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human skin fibroblast

Beschreibung der Zelllinie

Established from a biopsy of a normal 56 year old male. The cells have been used in radiation survival studies. EBV-transformed lymphocytes designated LB185 (Sigma Catalogue number. 98062318) have been isolated from the same individual. This has been confirmed by STR profiling at ECACC.

Anwendung

DNA repair studies

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 9
D5S818: 10,13
D7S820: 11
THO1: 8,9
TPOX: 8,10
vWA: 17,18

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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