CMT 93

89111413, mouse colon, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: CMT 93, 89111413
• Biologische Quelle: mouse colon
• Wachstumsmodus: Adherent
• Karyotyp: Hyperdiploid,modal no. 50
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse rectum carcinoma

Beschreibung der Zelllinie

From a 19 month old male mouse (C57BL/1CRF) which had received an i.p. injection of MAMA each week for 18 months. 4th in vivo passage - source of explant culture. Cells form acini and junctional complexes and produce large tumours in nude mice (6 x 1,000,000 cells inoculum) after 1 month.

Anwendung

Tumourigenicity studies

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.05% trypsin; 5% CO₂; 37°C. Cells grow in clumps.

Sonstige Hinweise

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Haftungsausschluss

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.