CCRF-SB

89090405, human blood, Lymphoblastoid

• UNSPSC-Code: 41106514

Eigenschaften

• product name: CCRF-SB, 89090405
• Biologische Quelle: human blood
• Wachstumsmodus: Suspension
• Karyotyp: 2n = 46
• Morphologie: Lymphoblastoid
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian acute lymphoblastic leukaemia

Beschreibung der Zelllinie

A B lymphoblastoid line obtained from the peripheral blood of an 11 year old boy with acute lymphoblastic leukaemia. Appear free of virus particles by electron microscopy. Do not synthesise immunoglobulins. Grown in continuous spinner culture for 6 months. HLA profile: A1, A2, B12, B17, Cw2.

Anwendung

HLA profile studies

Nährmedium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 3x10,000 to 1x100,000 cells/ml; 5% CO₂; 37°C. Cultures can tolerate high densities i.e. 1-2x1,000,000 cells/ml.

Sonstige Hinweise

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