HOS

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87070202, human bone, Epithelial-like

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human bone
• Verpackung: tube of 5 μg 87070202-DNA-5UG; pkg of vial of cells 87070202-1VL
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46, hyperdiploid
• Morphologie: Epithelial-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian osteosarcoma, TE85

Beschreibung der Zelllinie

Derived from a 13 year old female caucasian. Cells are sensitive to both virus and chemical transformation and exhibit a flat morphology, low saturation density and low plating efficiency in soft agar. HOS and 143B (Sigma Catalogue number 91112502) originated from the same host: 143B is TK- and originated indirectly from HOS which is TK+ (Skovgaard et al 2010 PMID: 20544518).

Anwendung

Virus, transformation and biochemical studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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