EIII

86072804, Human x Mouse Hybridoma hybrid, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: EIII, 86072804
• Biologische Quelle: Human x Mouse Hybridoma hybrid
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: pJ3.11, p79a, CFTR DNA markers
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human x mouse hybrid

Beschreibung der Zelllinie

This cell line may contain a sub-chromosomal fragment of human chromosome 17 bearing markers thought at the time to flank the cytotic fibrosis gene. The human DNA was donated from the human osteosarcoma cell line MNNG-HOS.

Anwendung

Somatic cell hybrid mapping

Nährmedium

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO₂.

Sonstige Hinweise

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