MM138 Cell Line human

10092321, human skin, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: MM138 Cell Line human, 10092321
• Biologische Quelle: human skin
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): metastasis
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human melanoma; from metastatic site - lymph node

Beschreibung der Zelllinie

Metastatic Melanoma (metastatic site: lymph node). Cell Bank Australia reference number: CBA1345.

Nährmedium

RPMI 1640 (with 2 mM L-Glutamine + 25 mM HEPES) + 10% fetal bovine serum (FBS).

Subkultur-Routine

Optimal split ratio 1:4, MM138 has a relatively slow doubling time of around 60 hours. It does not become confluent and will detach after 6-7 days. If split too hard the doubling time is greatly increased. (Seeding density 0.8 x10⁴ cells/cm²). Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min.

Sonstige Hinweise

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.