This item is a product of PromoCell. The manufacturer considers the formulation proprietary. However, this is a complete medium that includes all the growth factors and supplements necessary for providing optimal cell health and efficient adherence selection of monocytes from freshly isolated mononuclear cells. This medium does not contain antibiotics or antimycotics and is formulated for use in an incubator with an atmosphere of 5% CO2.
C-28051
Monocyte Attachment Medium
250 ml
Synonym(e):
Primary Cell Culture Media
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
Größe auswählen
Ansicht ändern
250 ML
CHF 136.00
About This Item
UNSPSC-Code:
12352207
NACRES:
NA.71
Form
aqueous solution
Verpackung
pkg of 250 ml
Methode(n)
cell culture | mammalian: suitable
Versandbedingung
wet ice
Lagertemp.
2-8°C
Allgemeine Beschreibung
Monocyte Attachment Medium (Ready-to-use)
Anwendung
The Monocyte Attachment Medium allows for the efficient adherence selection of monocytes from freshly isolated human mononuclear cells while maintaining optimal cell health. Thus, the time-consuming and costly immunomagnetic purification of monocytes is dispensable prior to experiments requiring adherent monocytes as a starting material. With properly performed washing steps large numbers of 80-90% pure monocytes can be obtained. Typical applications are generation of dendritic cells / macrophages as well as phagocytosis assays.Recommended for:Human mononuclear cells (freshly isolated)Human monocytes (freshly isolated)
Qualität
All lots of PromoCell Monocyte Attachment Medium are subjected to comprehensive quality control tests using primary human mononuclear cells from peripheral blood. Each lot of Monocyte Attachment Medium is checked for the efficient adherence selection of highly viable monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Angaben zur Herstellung
The Medium is chemically defined/xeno-free and does not need further supplementation.
Lagerung und Haltbarkeit
Store the Medium at 2 to 8°C in the dark immediately after arrival. Do not freeze the Medium. If stored properly, the product is stable until the expiry date stated on the label. After opening, the shelf life of the medium is 6 weeks at 2 to 8°C. For use, pre-warm only an aliquot of the medium and keep the remaining medium refrigerated at 2 to 8°C.
Subkultur-Routine
Click here for more information.
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Hier finden Sie alle aktuellen Versionen:
Analysenzertifikate (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Dokumente section.
Wenn Sie Hilfe benötigen, wenden Sie sich bitte an Kundensupport
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Artikel
Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.
Protokolle
Generierung von phagozytischen M1- und M2-Makrophagen aus humanen PBMC innerhalb von 10 Tagen in serum- und xenofreien Zellkulturmedien. Mehr als 350 PromoCell-Produkte entdecken.
Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.
Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.
-
Could you tell me the formula of this medium (C-28051)?
1 answer-
Helpful?
-
Active Filters
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.
