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Merck

77713

Sigma-Aldrich

Pefachrome® uPA

≥95.0% (HPLC)

Synonym(e):

N-Benzoyl-β-Ala-Gly-Arg-4-nitroanilid-acetat, N-Benzoyl-β-alanyl-glycyl-L-arginin-4-nitroanilid-acetat

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About This Item

Empirische Formel (Hill-System):
C24H30N8O6 · C2H4O2
CAS-Nummer:
Molekulargewicht:
586.60
MDL-Nummer:
UNSPSC-Code:
12352200
PubChem Substanz-ID:

Assay

≥95.0% (HPLC)

Form

powder

Löslichkeit

water: 5 mg/mL, clear, colorless

Lagertemp.

2-8°C

SMILES String

CC(O)=O.NC(=N)NCCC[C@H](NC(=O)CNC(=O)CCNC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C24H30N8O6.C2H4O2/c25-24(26)28-13-4-7-19(23(36)30-17-8-10-18(11-9-17)32(37)38)31-21(34)15-29-20(33)12-14-27-22(35)16-5-2-1-3-6-16;1-2(3)4/h1-3,5-6,8-11,19H,4,7,12-15H2,(H,27,35)(H,29,33)(H,30,36)(H,31,34)(H4,25,26,28);1H3,(H,3,4)/t19-;/m0./s1

InChIKey

LNYCMHUMNWBNSM-FYZYNONXSA-N

Anwendung

Pefachrome® substrates are used for chromogenic assays of various serine proteinases. Pefachrome® uPA is used for the detection of serine proteases such as urokinase-type plasminogen activator (urokinase).

Verpackung

1 Stück enthält 10 μmol Pefachrome® uPA und 20 mg Glycin

Sonstige Hinweise

Chromogenic substrate test for the detection of urokinase in Shigella[1]

Rechtliche Hinweise

Pefachrome is a registered trademark of Pentapharm AG, Basel

Lagerklassenschlüssel

13 - Non Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Lot/Batch Number

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Die Dokumentenbibliothek aufrufen

Rong Pan et al.
The Journal of biological chemistry, 285(30), 22950-22956 (2010-05-21)
Enzymatic catalysis of biochemical reactions is essential to all living systems. The "lock and key" and "induced fit" models were early contributions to our understanding of the mechanisms involved in the reaction between an enzyme and its substrate. However, whether
Urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-9 (MMP-9) expression and activity during early embryo development in the cow.
Whiteside EJ, Kan M, Jackson MM, et al.
Anat. Embryol., 204, 477-483 (2001)
M F Massenti et al.
Microbiologica, 10(3), 331-333 (1987-07-01)
A simple test for detection of urokinase in Shigella is described. The test is performed by suspending a loopful of bacteria in 100 microliters of a buffered 1mM solution of benzoyl-beta-alanyl glycyl-arginyl-4-nitroanilide acetate (Chromozym U). Enzymatic activity is revealed by
Yukari Yamamoto et al.
Journal of dermatological science, 47(2), 119-125 (2007-05-29)
Although pemphigus vulgaris (PV)-IgG has been shown to activate urokinase plasminogen activator (uPA) in cultured keratinocytes, activation of uPA is thought to have no primary role in PV-acantholysis, because PV-IgG is still pathogenic in uPA- and tissue-PA-knockout mice. To determine
D H Rhoney et al.
American journal of health-system pharmacy : AJHP : official journal of the American Society of Health-System Pharmacists, 56(20), 2047-2051 (1999-11-30)
The retention of urokinase activity after frozen storage was studied. Urokinase powder was reconstituted aseptically in sterile water for injection or preservative-free 0.9% sodium chloride injection to a final concentration of 5000 IU/mL. Samples were stored in 5-mL plastic syringes

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