66540024

RCi004-A

Human iPS Cell Line

• Synonym(e): EBiSC iPSC Line, Human iPSC, Induced Pluripotent Stem Cell Line, iPS Cell, iPSC
• UNSPSC-Code: 41106509

Eigenschaften

• Biologische Quelle: human dermis (fibroblast)
• Qualitätsniveau: 100
• Verfahren zur Umprogrammierung: episomal
• Beschreibung: age (N/A)
• Hersteller/Markenname: EBiSC^™
• Geschlecht: female
• Wachstumsmodus: adherent (pluripotent)
• Methode(n): cell culture | stem cell: suitable
• Relevante Krankheit(en): Huntington′s disease
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Allgemeine Beschreibung

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Ursprung der Zelllinie

Depositor
Roslin Cells Ltd.

Beschreibung der Zelllinie

Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: RCi004-B

Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)SSEA-1(-)TRA 1-60(+)SSEA-4 (+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:B-TUBULIN(-)FOXG1(+)HES5(+)NEUROD1(+)PAX6(+)Sox1(+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:CXCR4 (+)FOXA2(+)GSC(+)PITX1(+)SOX17(+)
Mesoderm:In vitro spontaneous differentiation
Marker Expressed: BMP4 (+)DCN(+)GATA4(+)HAND1(+)PDGF(+)PECAM(+)VIMENTIN(+)

Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
Karyotyping
Passage number: P16
Cell line karyotype: 46,XX,i(20)(q10)
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Verlinkung

• Additional Cell Line Info
• EBiSC Access Use Agreement (EAUA)
• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

Subkultur-Routine

Medium: mTeSR^®
Passage method: EDTA
Matrix: Matrigel^® / Geltrex^®
CO₂ concentration: 5%
O₂ concentration: 21%
Temperature: 37°C

Rechtliche Hinweise

EBiSC is a trademark of Fraunhofer-Gesellschaft

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

mTeSR is a registered trademark of WiCell Research Institute, Inc.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable