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P1254

Sigma-Aldrich

Brij® L23

suitable for Stein-Moore chromatography

Synonym(e):

Brij® 35, C12E23, Polyoxyethylen-23-laurylether

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500 G
CHF 65.60
5 KG
CHF 396.00

CHF 65.60


Voraussichtliches Versanddatum16. April 2025


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500 G
CHF 65.60
5 KG
CHF 396.00

About This Item

CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
12161900
PubChem Substanz-ID:
NACRES:
NA.21

CHF 65.60


Voraussichtliches Versanddatum16. April 2025


Bulk-Bestellung anfordern

Beschreibung

non-ionic

Qualitätsniveau

Form

solid

Mol-Gew.

micellar avg mol wt 48,000
estimated mol wt 1198

Aggregatnummer

20-40

CMC

91 μM

Übergangstemp.

cloud point >100 °C

HLB

16.9

Eignung

suitable for Stein-Moore chromatography

SMILES String

CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO

InChI

1S/C58H118O24/c1-2-3-4-5-6-7-8-9-10-11-13-60-15-17-62-19-21-64-23-25-66-27-29-68-31-33-70-35-37-72-39-41-74-43-45-76-47-49-78-51-53-80-55-57-82-58-56-81-54-52-79-50-48-77-46-44-75-42-40-73-38-36-71-34-32-69-30-28-67-26-24-65-22-20-63-18-16-61-14-12-59/h59H,2-58H2,1H3

InChIKey

IEQAICDLOKRSRL-UHFFFAOYSA-N

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Anwendung

Non-ionic detergent. Useful for the extraction of membrane proteins. Typically used in the range of 0.1-1%.

Rechtliche Hinweise

Brij is a registered trademark of Croda International PLC

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 2

Flammpunkt (°F)

>300.2 °F - open cup

Flammpunkt (°C)

> 149 °C - open cup

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Gloves


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Two forms of an acid proteinase have been purified from human erythrocyte membranes by a simple method involving selective extraction with 0.5% Brij 35, affinity chromatography on pepstatin A-Sepharose 4B, and chromatography on Sephacryl S-200 and DEAE-Sephadex G-100. One species
T Tobimatsu et al.
Archives of biochemistry and biophysics, 347(1), 132-140 (1997-11-05)
Recombinant adenosylcobalamin-dependent diol dehydratase of Klebsiella oxytoca overexpressed in Escherichia coli was purified to homogeneity. The enzyme has a low solubility and was extracted from the crude membrane fraction with 1% Brij 35 in a high recovery. Subsequent chromatography on
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Questions

  1. How to prepare a 1% solution of Brij 35?

    1 answer
    1. To prepare a 1% solution of Brij 35, first verify the concentration of the Brij 35 solution. Once the concentration is known, can use water as the diluent to prepare the 1% solution of Brij 35. If the Brij 35 solution is not already at the desired 1% concentration, dilute it accordingly with water.

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