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Merck

75917

Millipore

Hirn-Herz-Infusion, Schwein

suitable for microbiology

Synonym(e):

BHI Broth, Brain Heart Broth

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About This Item

UNSPSC-Code:
41106212
NACRES:
NA.85

Biologische Quelle

Porcine heart

Qualitätsniveau

Form

powder

Haltbarkeit

limited shelf life, expiry date on the label

Zusammensetzung

amino-nitrogen, ≥4.5%
total nitrogen (N), ≥11%

Verpackung

pkg of 500 g

Lagerbedingungen

(Tightly closed. Dry.)

Glührückstand

≤17%

Verlust

≤6.0% loss on drying

Farbe

light yellow

pH-Wert

6.5-7.5 (25 °C, 2% in H2O)

Löslichkeit

H2O: 2%, clear, light yellow (and light brown-yellow and light brown)

Anwendung(en)

food and beverages
microbiology

Allgemeine Beschreibung

Brain Heart Infusion (BHI), Porcine, is a culture medium commonly used in microbiology for the cultivation of various fastidious, pathogenic microorganisms, including yeasts and molds. The media is nutritious and well-buffered to support the growth of a wide range of microorganisms such as Streptococci, Pneumococci, Meningococci, etc. The media is especially suited for the cultivation of Staphylococci for the plasma coagulase test and setting up blood cultures.BHI is typically prepared using porcine (pig) brain and heart tissues as the source of the raw materials. The enzymatic digestion of porcine brain-heart infusion provides amino acids, nitrogen, carbon, vitamins, and minerals for the growth of organisms. Glucose is the source of carbohydrates, and sodium chloride maintains the osmotic balance of the medium. Disodium phosphate is a buffering agent.

Anwendung

Brain Heart Infusion, porcine, is recommended for the cultivation of fastidious pathogenic microorganisms and is often used for the examination of water, wastewater, meat, and food products. It is used as a culture medium for the growth of Streptococcus pneumonia, Listeria monocytogenes, Leishmania promastigotes, and Clostridium difficile.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.

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