QIA92

Caspase Detection Kit (Red-VAD-FMK)

• UNSPSC-Code: 41116133

Eigenschaften

• Verwendung: sufficient for 100 tests
• Qualitätsniveau: 100
• Speziesreaktivität (Voraussage durch Homologie): mammals
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Methode(n): flow cytometry: suitable
• Aufnahme: sample type intact cells
• Fluoreszenz: λ_ex ~540 nm; λ_em ~570 nm
• Nachweisverfahren: fluorometric
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

A convenient and sensitive method for the detection of activated caspases in living cells. The fluorescent marker is VAD-FMK conjugated to sulforhodamine. This cell-permeable, non-toxic inhibitor binds irreversibly to activated caspases in apoptotic cells. The fluorescence intensity can be measured by fluorescence microscopy, fluorescence microplate reader or flow cytometry.

The activation of caspases plays a central role in apoptosis. The Caspase Detection Kit provides a convenient and sensitive means for detecting activated caspases in situ in living cells. The assay utilizes a caspase inhibitor (VAD-FMK) conjugated to sulfo-rhodamine as the fluorescent in situ marker. Red-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells. The red fluorescence label allows for direct detection of activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.

Spezifität

A broad range of species

Komponenten

Labeled Caspase Inhibitor (Red-VAD-FMK), Wash Buffer, Caspase Inhibitor (Z-VAD-FMK), and a user protocol

Warnhinweis

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Spezifikationen

Assay Time: 1.5 h

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids