MABN2487

Anti-Astrin Antibody, clone C-1

clone C-1, from mouse

• Synonym(e): Sperm-associated antigen 5, Deepest, Mitotic spindle-associated protein p126, MAP126
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: C-1, monoclonal
• Speziesreaktivität: human
• Verpackung: antibody small pack of 25 μg
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_006452
• UniProt-Hinterlegungsnummer: Q96R06
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... SPAG5(10615)

Beschreibung

Allgemeine Beschreibung

Sperm-associated antigen 5 (UniProt: Q96R06; also known as Astrin, Deepest, Mitotic spindle-associated protein p126, MAP126) is encoded by the SPAG5 gene (Gene ID: 10615) in human. Astrin is a homodimeric protein with a globular head domain and a long stalk. It is an essential component of the mitotic spindle. Astrin is highly expressed in testis and is detected at low levels in placenta, liver, pancreas, thymus and colon. Its expression is cell cycle-regulated, with an increase from prophase to cytokinesis and return to basal levels at the next G1 phase. In non-dividing cells Astrin is associated with microtubules unless under cellular stress, then it is localized in stress granules. In non-mitotic cells, upon stress induction, it inhibits mTORC1 association and recruits the mTORC1 component RPTOR to stress granules, thereby preventing mTORC1 hyperactivation-induced apoptosis. Astrin is required for normal chromosome segregation and progression into anaphase. It is also required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. In complex with SKAP, it promotes stable microtubule-kinetochore attachments. Depletion of astrin in cells is shown to result in the activation of p53-dependent apoptosis that elevates Bax expression and increases Caspase-3 activity. Astrin can be phosphorylated at multiple sites by Aurora kinase A. (Ref.: Yang, YC., et al. (2006). Biochem. Biophys. Res. Commun. 343(2); 428-434).

Spezifität

Clone C-1 specifically detects human Sperm-associated antigen 5 (Astrin). It detects both splice variants (130 and 140 kDa).

Immunogen

His-tagged recombinant fragment corresponding to the first 952 amino acids from human Sperm-associated antigen 5 (Astrin).

Anwendung

Research Category
Neurowissenschaft

Anti-Astrin, clone C-1, Cat. No. MABN2487, is a highly specific mouse monoclonal antibody that targets Sperm-associated antigen 5 and has been tested for use in Immunocytochemistry and Western Blotting.

Western Blotting Analysis: A representative lot detected Astrin in Western Blotting applications (Yang, Y.C., et. al. (2006). Biochem Biophys Res Commun. 343(2):428-34).

Immunocytochemistry Analysis: A representative lot detected Astrin in Immunocytochemistry applications (Schmidt, J.C., et. al. (2010). J Cell Biol. 191(2):269-80; Yang, Y.C., et. al. (2006). Biochem Biophys Res Commun. 343(2):428-34; Kern, D.M., et. al. (2016). J Cell Biol. 213(3):315-28).

Qualität

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Astrin in HeLa cell lysate.

Zielbeschreibung

~130 and 140 kDa doublet; 134.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.