MABD127

Anti-FBXO15 Antibody, clone 3B11.1

clone 3B11.1, from mouse

• Synonym(e): F-box only protein 15
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 3B11.1, monoclonal
• Speziesreaktivität: human
• Methode(n): western blot: suitable
• Isotyp: IgMκ
• NCBI-Hinterlegungsnummer: NP_001136430
• UniProt-Hinterlegungsnummer: Q8NCQ5
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... FBXO15(201456)

Beschreibung

Allgemeine Beschreibung

F-box only protein 15 (UniProt Q8NCQ5) is encoded by the FBXO15 (also known as FBX15) gene (Gene ID 201456) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, U-box-type, and PHD-finger-type E3 ligases. The RING finger-type E3s include seven cullin-based E3s, such as SKP1–CUL1–F-box-protein (SCF) complex and the anaphase-promoting complex/cyclosome (APC/C). F-box proteins use their N-terminal F-box motif to bind Skp1 and other motif(s) in their C-terminal region for substrates recognition and recruitment. F-box proteins are divided into the FBXW subclass that contains WD40 repeat domains, the FBXL subclass that contains leucine-rich repeat domains, and the F-box only (FBXO) subclass that contains not yet fully characterized substrate recognition domains. SCF-Fbxo15 is reported to mediate the ubiquitination of cardiolipin synthase (CLS1) for proteasomal degradation in human lung epithelial cells. PTEN-induced putative kinase 1 (PINK1) is shown to phosphorylate CLS1 and cause its release from the inner mitochondrial membrane to the outer membrane, making it accessible for Fbxo15-targeted ubiquitination.

Spezifität

Clone 3B11.1 recoginzes an N-terminal epitope present in spliced isoform 1 (UniProt Q8NCQ5-1), but absent in spliced isoform 2 (UniProt Q8NCQ5-2).

Immunogen

Epitope: N-terminal region.

KLH-conjugated linear peptide corresponding an N-terminal sequence of human FBXO15.

Anwendung

Research Category
Stammzellforschung

Research Sub Category
Ubiquitin & Ubiquitin-Stoffwechsel

Anti-FBXO15 Antibody, clone 3B11.1 is an antibody against FBXO15 for use in Western Blotting.

Qualität

Evaluated by Western Blotting in human H9 embryonic stem cell lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected FBXO15 in 10 µg of human H9 embryonic stem cell lysate.

Zielbeschreibung

~57 kDa observed. 57.26 kDa calculated. Uncharacterized band(s) may appear in some lysates.

Physikalische Form

Format: Purified

Purified mouse monoclonal IgMκ antibody in buffer containing PBS with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable