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ABE2973

Sigma-Aldrich

Anti-CREB-H/CREB3L3

Synonym(e):

Cyclic AMP-responsive element-binding protein 3-like protein 3, Transcription factor CREB-H, cAMP-responsive element-binding protein 3-like protein 3

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Mol-Gew.

calculated mol wt 49.08 kDa
observed mol wt ~45 kDa

Aufgereinigt durch

using Protein A

Speziesreaktivität

mouse

Speziesreaktivität (Voraussage durch Homologie)

human

Verpackung

antibody small pack of 100

Methode(n)

ChIP: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgG

Epitopsequenz

Internal

Protein-ID-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Lagertemp.

-10 to -25°C

Angaben zum Gen

human ... CREB3L3(84699)

Spezifität

This rabbit polyclonal antibody detects CREB-H (CREB3L3). It targets an epitope within the internal region.

Immunogen

GST/His-tagged recombinant fragment corresponding to 92 amino acids from the internal region of human Cyclic AMP-responsive element-binding protein 3-like protein 3 (CREB-H).

Anwendung

Quality Control Testing

Evaluated by Western Blotting in lysate from wild-type Mouse liver tissue.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected CREB-H in lysate from wild-type Mouse liver tissue, but not in lysate from Mouse liver with CREB-H knockout.

Tested Applications

Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected CREB-H/CREB3L3 in Chromatin Immunoprecipitation applications. (Zhang, K., et al. (2006). Cell. 124(3); 587-599).

Western Blotting Analysis (WB): A representative lot detected CREB-H/CREB3L3 in Western Blotting applications. (Kim, H., et al. (2014). Endocrinology. 155(3); 769-782).

Immunohistochemistry Applications: A representative lot detected CREB-H/CREB3L3 in Immunohistochemistry applications. (Kim, H., et al. (2014). Endocrinology. 155(3); 769-782).

Immunofluorescence Analysis (IF): A representative lot detected CREB-H/CREB3L3 in Immunofluorescence applications ( (Zhang, K., et al. (2006). Cell. 124(3); 587-599).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CREB-H/CREB3L3 in Immunoprecipitation applications. (Zhang, K., et al. (2006). Cell. 124(3); 587-599).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Zielbeschreibung

Cyclic AMP-responsive element-binding protein 3-like protein 3 (UniProt: Q68CJ9; also known as cAMP-responsive element-binding protein 3-like protein 3, Transcription factor CREB-H) is encoded by the CREB3L3 (also known as CREBH, HYST1481) gene (Gene ID: 84699) in human. CREB-H is a single-pass type II membrane protein that is exclusively expressed in the liver. It contains a cytoplasmic domain (aa 1-322), a transmembrane domain (aa 323-343), and a lumenal domain (aa 344-461). It is a RIP-regulated liver-specific transcription factor that is cleaved upon endoplasmic reticulum (ER) stress and is required to activate expression of acute phase response genes. In response to ER stress, CREB-H is cleaved by site-1 and site-2 proteases to liberate an N-terminal fragment that transits to the nucleus to activate transcription of the genes encoding serum amyloid P-component and C-reactive protein. Proinflammatory cytokines are also shown to increase the expression of ER membrane-anchored CREB-H. It is activated in response to cAMP stimulation and is reported to bind to the cAMP response element (CRE) and box-B element. It plays a crucial role in the regulation of triglyceride metabolism and is required for the maintenance of normal plasma triglyceride levels. CREB-H is shown to interact with PPAR to regulate FGF21, a hepatic hormone that regulates whole-body energy homeostasis. CREB-H and PPAR are reported to act as interactive trans-activators that regulate each other for their expression. They form a functional complex upon fasting or the atherogenic high-fat diet. CREB-H-deficient mice display hypertriglyceridemia secondary to inefficient triglyceride clearance catalyzed by lipoprotein lipase. (Ref.: Kim, H., et al. (2014). Endocrinology. 155(3);769-782; Lee, JH., et al. (2011). Nat. Med. 17(7); 812-815; Zhang, K., et al. (2006). Cell. 124(3); 587-599).

Physikalische Form

Purified rabbit polyclonal antibody in PBS with 50% glycerol and without azide.

Rekonstituierung

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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