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ABE2922

Sigma-Aldrich

Anti-Histone H1E frameshift mutant

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise

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25 μG
CHF 150.00
100 μG
CHF 377.00

CHF 150.00


Voraussichtliches Versanddatum16. April 2025



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25 μG
CHF 150.00
100 μG
CHF 377.00

About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

CHF 150.00


Voraussichtliches Versanddatum16. April 2025


Biologische Quelle

rabbit

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Mol-Gew.

calculated mol wt 21.86 kDa
observed mol wt ~33 kDa

Aufgereinigt durch

affinity chromatography

Speziesreaktivität

human

Verpackung

antibody small pack of 100 μg

Methode(n)

affinity binding assay: suitable
immunofluorescence: suitable
western blot: suitable

Isotyp

IgG

Epitopsequenz

Unknown

Protein-ID-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

2-8°C

Posttranslationale Modifikation Target

mutation (Frameshift aa145-150)

Angaben zum Gen

human ... Unknown(3008)

Allgemeine Beschreibung

Histone 1, H1e (UniProt: A3R0T8; also known as Histone H1.4, HIST1H1E) is encoded by the HIST1H1E (also known as hCG_16457421) gene (Gene ID: 3008) in human. Histones have been classified under five major classes. The core histones H2A, H2B, H3, and H4 constitute the protein components of nucleosome core particles and histone H1 is a linker histone that plays a role in chromatin folding. Depletion of histone H1 can cause dramatic changes in chromatin structure, global nucleosome spacing, and reduced chromatin compaction. Pathogenic mutations in HIST1H1E gene result in the formation of histone H1 that is less effective in neutralizing negatively charged linker DNA and display reduced DNA binding and protein-protein interactions due to truncation in key residues. These mutations are reported to result in a shift in reading frame in the C-terminal region and produce truncated proteins. Frameshift mutations in HIST1H1E have been linked to Rahman syndrome that is manifested in overgrowth with intellectual disabilities (OGID) in early infancy. Subjects with Rahman syndrome display a global tendency in reduction of methylation state. (Ref.: Andrea Ciolfi, et al. (2020). Clinical Epigenetics 12; Article 7; Tatton-Brown , K., et al. (2017). Am. J. Hum. Genet. 100(5); 725-736).

Spezifität

This rabbit polyclonal antibody specifically detects Histone H1 with H1E frameshift mutation.

Immunogen

KLH-conjugated Linear Peptide corresponding to the Unknown of Human Histone H1.

Anwendung

Quality Control Testing

Evaluated by Western Blotting in human oligodendroglioma cell line SCC163 stably expressing transgenic H1Efs-FLAG-HA.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Histone H1E frameshift mutant in human oligodendroglioma cell line SCC163 stably expressing transgenic H1Efs-FLAG-HA.

Tested Applications

Immunofluorescence Analysis: A representative lot detected Histone H1E frameshift mutant in mESc and H1Eframeshift Knock-in cells (Courtesy of Prof. C. David Allis Lab at Rockefeller University).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Histone H1E frameshift mutant in Human WBC and mESC′s & various other cell lines (Courtesy of Prof. C. David Allis Lab at Rockefeller University).

Affinity Binding Assay: A representative lot of this antibody bound Histone H1E peptide with a KD of 1.0 x 10-12

in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Histone H1E frameshift mutant, Cat. No. ABE2922, is a rabbit polyclonal antibody that detects Histone H1E frameshift mutant and is tested for use in Affinity Binding Assay, Immunofluorescence, and Western Blotting.

Physikalische Form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1


Analysenzertifikate (COA)

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