ABE149

Anti-ASF1a Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Histone chaperone ASF1A, Anti-silencing function protein 1 homolog A, hAsf1, hAsf1a, CCG1-interacting factor A, CIA, hCIA
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IP, WB
• Citations: 3

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human, rat
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_054753
• UniProt accession no.: Q9Y294
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ASF1A(25842)

Beschreibung

General description

ASF1a (anti-silencing function 1a) is a histone H3/H4 chaperone and chromatin regulator. This protein facilitates histone placement and histone removal and exchange during nucleosome assembly/disassembly and is essential for the formation of senescence-associated heterochromatic foci (SAHF). ASF1a interacts with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA inside PML bodies of cells approaching senescence after p53/p21 activation.

~23 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to human ASF1a.

Application

Anti-ASF1a Antibody is a Rabbit Polyclonal Antibody for detection of ASF1a also known as Histone chaperone ASF1A or hAsf1 & has been validated in WB & IP.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Banumathy, G, et al. (2009). Mol Cell Biol. 29(3):758–770.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected ASF1a on 10 µg of HeLa cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable