ABE1049

Anti-NRF-1 Antibody

serum, from goat

• Synonym(e): Nuclear respiratory factor 1, Alpha-pal, Alpha palindromic-binding protein, NRF-1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Klon: polyclonal
• application: EMSA; WB
• Speziesreaktivität: human
• Methode(n): electrophoretic mobility shift assay: suitable; western blot: suitable

Eigenschaften

• Biologische Quelle: goat
• Qualitätsniveau: 100
• Antikörperform: serum
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: human
• Methode(n): electrophoretic mobility shift assay: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_001035199
• UniProt-Hinterlegungsnummer: Q16656
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... NRF1(4899)

Beschreibung

Allgemeine Beschreibung

Nuclear respiratory factor 1 (UniProt Q16656; also known as Alpha-pal, Alpha palindromic-binding protein, NRF-1) is encoded by the NRF1 (Also known as ALPHA-PAL) gene (Gene ID 4899) in human. NRF-1 is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. NRF-1 is upregulated and activated/phosphorylated by Akt in response to oxidant exposure or increased cellular reactive oxygen species (ROS), reulsting in NRF-1 nuclear translocation, where it binds to DNA as a homodimer and mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.

Spezifität

Expected to react with all four spliced isoforms of human NRF-1 reported by UniProt (Q16656). This antiserum bound DNA-NRF-1 complex as evidenced by a specific supershift of NRF-1, but not NRF-2, DNA complex in EMSA (Virbasius, C.A., et al. (1993). Genes Dev. 7(12A):2431-2445).

Immunogen

Recombinant human NRF-1.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Transkriptionsfaktoren

Anti-NRF-1 Antibody is an antibody against NRF-1 for use in Western Blotting, Electrophoretic Mobility Shift Assay.

Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of DNA-NRF-1 complex in EMSA using radiolabeled oligonucleotide containing human mitochondrial transcription specificity factor genes (TFB1M & TFB2M) NRF-1-binding site and HeLa nuclear extract (NE) or purified recombinant NRF-1 (Gleyzer, N., et al. (2005). Mol. Cell. Biol. 25(4):1354-1366).
Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of DNA-NRF-1 complex in EMSA using radiolabeled oligonucleotide containing rat cytochrome C NRF-1-binding site and HeLa nuclear extract (NE), purified HeLa NRF-1 or recombinant NRF-1. No shift by this antiserum of HeLa NE proteins complexed with oligonucleotide containing mouse COXVb gene NRF-2-binding site was observed (Virbasius, C.A., et al. (1993). Genes Dev. 7(12A):2431-2445).

Qualität

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: An 1:200 dilution of this antibody detected NRF1 in 10 µg of HeLa nuclear extract.

Zielbeschreibung

~68 kDa observed. 53.54 kDa (isoform Long), 46.16 kDa (isoform Short), 36.05 kDa (isoform 3), 55.67 kDa (isoform 4) calculated.

Physikalische Form

Unpurified

Goat polyclonal serum with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable