ABC227

Anti-GABARAPL2

from rabbit, purified by affinity chromatography

• Synonym(e): Gamma-aminobutyric acid receptor-associated protein-like 2, GABA(A) receptor-associated protein-like 2, Ganglioside expression factor 2, GEF-2, General protein transport factor p16, Golgi-associated ATPase enhancer of 16 kDa, GATE-16, MAP1 light chain 3-
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Verpackung: antibody small pack of 25 μL
• Methode(n): immunohistochemistry: suitable (paraffin); western blot: suitable
• UniProt-Hinterlegungsnummer: P60520
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... GABARAPL2(11345)

Beschreibung

Allgemeine Beschreibung

Gamma-aminobutyric acid receptor-associated protein-like 2 (UniProt: P60520; also known as GABA(A) receptor-associated protein-like 2, Ganglioside expression factor 2, GEF-2, General protein transport factor p16, Golgi-associated ATPase enhancer of 16 kDa, GATE-16, MAP1 light chain 3-related protein) is encoded by the GABARAPL2 (also known as FLC3A, GEF2) gene (Gene ID: 11345) in human. GEF-2 is a momomeric ubiquitin-like modifier protein of ATG8 family that is involved in intra-Golgi traffic. It is shown to modulate intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF, which in turn stimulates the association with GOSR1. GEF-2 is also involved in autophagy and plays a role in mitophagy, which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level. It is ubiquitously expressed and higher expressions are observed in the brain, heart, prostate, ovary, spleen and skeletal muscle. Low expression levels are observed in lung, thymus, and small intestine. In the precursor molecule the peptide bond between aa 116 and 117 is cleaved off by ATG4 to generate mature protein.

Spezifität

This rabbit polyclonal antibody detects GABA(A) receptor-associated protein-like 2 (GABARAPL2) in human cells.

Immunogen

GST-tagged full length human recombinant GABA(A) receptor-associated protein-like 2 (GABARAPL2).

Anwendung

Anti-GABARAPL2, Cat. No. ABC227, is a rabbit polyclonal antibody that detects Gamma-aminobutyric acid receptor-associated protein-like 2 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected GABARAPL2 in human cerebellum and human prostate tissue sections.

Qualität

Evaluated by Western Blotting in Raji membrane extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected GABARAPL2 in Raji membrane extract.

Zielbeschreibung

~ 14 kDa observed; 13.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide/.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.