AB3121

Anti-Angiopoietin-2 Antibody, NT

Chemicon^®, from rabbit

• Synonym(e): Ang-2
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: mouse
• Hersteller/Markenname: Chemicon^®
• Methode(n): ELISA: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NM_001147.1
• UniProt-Hinterlegungsnummer: O15123
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Angpt2(11601)

Beschreibung

Spezifität

Recognizes mouse Angiopoietin-2 (Ang-2). Mouse/human Ang-2 is a 496 AA secreted angiogenic protein (Suri et al. 1996). There is ~85% homology between mouse and human Ang-2. It is only 60% homologous with Ang-1 (Davis et al. 1996). Ang-2 may act as an antagonist of Ang-1 and Tie-2 (Suri et al. 1996). It is prominently expressed in tissues that are in involved in vascular remodeling (ovary, placenta, and uterus).

The immunogenic peptide sequence is 71% (15/21 aa) homologous with human Ang-2 and has no substantial homology with Ang-1. Antibody crossreactivity in other species is not established. The immunogen peptide is 85% conserved in porcine and 55% in chicken.

Immunogen

20 amino acid peptide sequence near the amino-terminus of mouse Ang-2.

Epitope: N-terminus

Anwendung

Research Category
Zellstruktur

Research Sub Category
ECM-Proteine

Detect Angiopoietin-2 using this Anti-Angiopoietin-2 Antibody, N-terminus validated for use in ELISA & WB.

Western blot: 1-10 μg/mL using ECL. The predicted size of Ang-2 is ~55 kDa. Like Ang-1, its size may higher due to glycosylation.

ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide per well.

Optimal working dilutions must be determined by end user.

Physikalische Form

Affinity purified immunoglobulin presented as liquid in PBS containing 0.1% BSA and 0.05% azide, pH 7.5

Lagerung und Haltbarkeit

Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. May be stored at 2-8°C for short term use. Avoid repeated freeze/thaw cycles.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable