616430

TGF-α, Human, Recombinant, E. coli

• Synonym(e): TGF-α, Human, Recombinant, E. coli, Transforming Growth Factor-α
• CAS-Nummer: 105186-99-0
• MDL-Nummer: MFCD00082077
• UNSPSC-Code: 12352202

Eigenschaften

• Assay: ≥97% (SDS-PAGE)
• Qualitätsniveau: 100
• Form: lyophilized
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Verunreinigungen: ≤100 pg/μg Endotoxin (pg/μg TGF-α)
• Versandbedingung: dry ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Recombinant, human transforming growth factor-α expressed in E. coli. Plays an important role in cell-cell adhesion and cell growth and differentiation. Reported to play a role in the development of hormonal insensitivity in estrogen-receptor-positive breast cancers.

Biochem./physiol. Wirkung

ED₅₀= 0.1-0.4 ng/ml as measured by the ability to stimulate ³H-thymidine incorporation in the mouse fibroblast cell line Balb/3T3

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

Lyophilized from 30% acetonitrile, 0.1% TFA.

Rekonstituierung

Following reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml with sterile 10 mM acetic acid containing ≥0.1% HSA or BSA.

Sonstige Hinweise

Nicholson, R.I., et al. 1994. Cancer Res. 54, 1684.
Anklesaria, P., et al. 1990. Proc. Natl. Acad. Sci. USA87, 3289.
Massague, J. 1990. J. Biol. Chem.265, 21393.
Watanabe, S., et al. 1987. Proc. Natl. Acad. Sci. USA84, 1258.
Schreiber, A.B., et al. 1986. Science232, 1250.
Tam, J.P. 1985. Science229, 673.
Derynck, R., et al. 1984. Cell38, 327.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany